Flanking Sequence Tags (FSTs) have been generated for rice insertional mutational populations. These FSTs are the flanking genomic sequence adjacent to the insertion and are obtained by TAIL PCR. We downloaded the FSTs from 8 different groups on June 12, 2008 and mapped 58,023 sequences onto version 6 of the Rice Genome Annotation Project pseudomolecules using the GMAP alignment program. Valid alignments are required to exceed 95% identity over 90% of the length.

The three main insertional mutations present in this list are:
-T-DNA: These insertions generated by random Agrobacterium mediated insertion of a selectable marker
-Tos17:This is a retroelement that accumulates in euchromatin during tissue culture
-Ac/Ds:A heterogous Ac/Ds (transposon tagging) system has been generated in rice by transformation

Additionally, there are four different ways to search or identify insertions into the genome. The first interface allows the user to define the chromosome, physical distance on the pseudomolecule, and type of insertion. The second interface allows the user to search for all FSTs in a particular BAC or PAC. The third interface allows the user to search for the physical position of an FST marker using the FST name. The fourth interface allows the user to identify the physical position of the FST by using the GenBank accession.

For the mapped FST sequences enter the rice locus identifer below to screen for an FST insertion(s) at the corresponding locus:
(e.g., LOC_Os01g01030)

Choose chromosome, FST type and physical interval on chromosome:

To obtain FST information for a particular BAC or PAC:
(e.g., OSJNBb0058P18, P0037C04, and OJ1529_G03):

To obtain physical position for a particular FST by name:
(e.g. DAM2B11, Ds264):

To obtain physical and genetic mapping information for a particular FST by GenBank accession:
(e.g. AG025435 or CL517450)